BioInnovatise

BioInnovatise specializes in fast, reliable, and high-quality CDMO services for plasmid DNA and viral vector-based research in early discovery phases of cell and gene therapy, immunotherapy, oncology, precision medicine, regenerative medicine, stem cell research, and vaccine development.

BioInnovatise is a growing, minority-owned biotechnology company that serves research and development laboratories in the I-270 Technology Corridor, BioCapital, and beyond. Our teams advise and produce custom projects that further the research of numerous startups, large pharmaceutical companies, academic labs, and government agencies, including the NIH and FDA. We specialize in molecular biology products and services to advance research in the early discovery phases of cell and gene therapy, immunotherapy, oncology, precision medicine, regenerative medicine, stem cell research, and vaccine development.

Our mission is to accelerate scientific discovery by providing fast, reliable, and high-quality CDMO services for plasmid DNA and viral vector-based research. We collaborate with researchers in government, academia, and industry to keep projects on track and drive innovation in cell and gene therapy, immunotherapy, precision medicine, and more.

Plasmid DNA Services:

Molecular Cloning Service: Customized cloning solutions with a standard turnaround time of 14 days.

Mutagenesis Service: Includes PCR site-directed mutagenesis, random mutagenesis, and transposon mutagenesis, all completed within 7 to 14 days.

Library Construction Services: Custom library construction requests on DNA sequences where more precision and large sequence volume is required. Specific mutations can be achieved using oligonucleotide based, overlap extension PCR, or synthetic oligonucleotide techniques within 7 to 14 days.

Disease-Related and Mutant Clones: In-stock clones for to specific research needs.

Plasmid DNA Preparation and Purification Services: Including midiprep (100 μg) in 7 days, maxiprep (1-2 mg) in 14 days, and gigaprep large-scale volumes available upon request.

Viral Vector Packaging:

Adenovirus Packaging: Volumes ranging from 250 μl – 1 ml with titer levels of 10E11 GC/ml - 10E13 GC/ml within 7 – 28 days depending on volume and titration requirements.

AAV Packaging: Volumes ranging from 250 μl – 1 ml with titer levels of 10E11 IFU/ml - 10E12 IFU/ml within 7 – 28 days depending on volume and titration requirements.

Lentivirus Packaging: Volumes ranging from 250 μl – 1 ml with titer levels of 10E8 TU/ml - 10E9 TU/ml within 7 – 28 days depending on volume and titration requirements.

Retrovirus Packaging: Volumes ranging from 250 μl – 1 ml with titer levels of 10E8 TU/ml - 10E9 TU/ml within 7 – 28 days depending on volume and titration requirements.

CRISPR-Cas9 Services:

CRISPR Vector Library or Custom Production: 5 pre-made vector designs or custom productions for targeted gene editing within 14 days

CRISPR Cassette Production and Donor Vector Construction Design: 3 pre-made vector cassettes for vectors or custom productions including Cas nuclease and gRNA design within 14 days.

sgRNA Cloning: Designing, generating, and cloning sgRNAs for CRISPR-Cas9 gene-editing experiments within 7 – 17 days.

CRISPR-Cas9 Knock-In or Knock-Out Service: A whole product solution for researchers to knock out or knock-in a gene of interest within 4 months.

Feeder Cells For Stem Cell Research / Primary Mouse Embryonic Fibroblasts

Primary Mouse Embryonic Fibroblasts: 6 options derived directly from embryonic tissue rather than being passaged or cultured for an extended period. The cell selection of fibroblast cells is isolated from mouse embryos every 12.5 days. Produced within 3 weeks.